cleaved parp 1 Search Results


cleaved parp 1  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology cleaved parp 1
Cleaved Parp 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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parp1  (ABclonal Biotechnology)
94
ABclonal Biotechnology parp1
A The correlation analysis between TKT and γH2AX expression using the published proteomic and phosphoproteomics dataset of 159 HCC patients from Fudan University. B The expression levels of γH2AX in TKT high and low tissues from 6 patients with HCC were compared using WB. C Representative MIF staining micrographs showing TKT (low or high) and γH2AX expression in an HCC microarray. Scale bar, 200 μm (TKT, γH2AX, DAPI and Merged) and 20 μm (Enlarged). D The Hep3B (Left) and HCCLM3 (Right) cells were collected without treatment, or at 2 h, 6 h, 12 h and 24 h post 4 Gy X-Ray treatment with siCtrl or siTKT transfection. Cell lysates were immunoblotted with the indicated antibodies. M, protein ladder. E Representative fluorescence images (green, γH2AX; blue, DAPI) and quantification of γH2AX immunostaining in cells at the indicated time after X-Ray treatment with or without TKT depletion. The correlation analysis between TKT and RAD51 ( F ), <t>PARP1</t> ( G ), XRCC6 ( H ), XRCC4 ( I ) expression in HCC tissues by Spearman correlation analysis from TCGA database. Adapted from GEPIA: http://gepia.cancer-pku.cn/ . TPM, transcripts per million.
Parp1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cleaved+parp+1/%5BKO+Validated%5D+PARP1+Rabbit+pAb/pmc10890932-271-4-20
Average 94 stars, based on 1 article reviews
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rabbit anti cleaved parp1 p25  (ABclonal Biotechnology)
94
ABclonal Biotechnology rabbit anti cleaved parp1 p25
A The correlation analysis between TKT and γH2AX expression using the published proteomic and phosphoproteomics dataset of 159 HCC patients from Fudan University. B The expression levels of γH2AX in TKT high and low tissues from 6 patients with HCC were compared using WB. C Representative MIF staining micrographs showing TKT (low or high) and γH2AX expression in an HCC microarray. Scale bar, 200 μm (TKT, γH2AX, DAPI and Merged) and 20 μm (Enlarged). D The Hep3B (Left) and HCCLM3 (Right) cells were collected without treatment, or at 2 h, 6 h, 12 h and 24 h post 4 Gy X-Ray treatment with siCtrl or siTKT transfection. Cell lysates were immunoblotted with the indicated antibodies. M, protein ladder. E Representative fluorescence images (green, γH2AX; blue, DAPI) and quantification of γH2AX immunostaining in cells at the indicated time after X-Ray treatment with or without TKT depletion. The correlation analysis between TKT and RAD51 ( F ), <t>PARP1</t> ( G ), XRCC6 ( H ), XRCC4 ( I ) expression in HCC tissues by Spearman correlation analysis from TCGA database. Adapted from GEPIA: http://gepia.cancer-pku.cn/ . TPM, transcripts per million.
Rabbit Anti Cleaved Parp1 P25, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti caspase papr  (ABclonal Biotechnology)
94
ABclonal Biotechnology anti caspase papr
A The correlation analysis between TKT and γH2AX expression using the published proteomic and phosphoproteomics dataset of 159 HCC patients from Fudan University. B The expression levels of γH2AX in TKT high and low tissues from 6 patients with HCC were compared using WB. C Representative MIF staining micrographs showing TKT (low or high) and γH2AX expression in an HCC microarray. Scale bar, 200 μm (TKT, γH2AX, DAPI and Merged) and 20 μm (Enlarged). D The Hep3B (Left) and HCCLM3 (Right) cells were collected without treatment, or at 2 h, 6 h, 12 h and 24 h post 4 Gy X-Ray treatment with siCtrl or siTKT transfection. Cell lysates were immunoblotted with the indicated antibodies. M, protein ladder. E Representative fluorescence images (green, γH2AX; blue, DAPI) and quantification of γH2AX immunostaining in cells at the indicated time after X-Ray treatment with or without TKT depletion. The correlation analysis between TKT and RAD51 ( F ), <t>PARP1</t> ( G ), XRCC6 ( H ), XRCC4 ( I ) expression in HCC tissues by Spearman correlation analysis from TCGA database. Adapted from GEPIA: http://gepia.cancer-pku.cn/ . TPM, transcripts per million.
Anti Caspase Papr, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cleaved+parp+1/%5BKO+Validated%5D+PARP+Rabbit+mAb/pm38996786-111-46-49
Average 94 stars, based on 1 article reviews
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cleaved poly  (Elabscience Biotechnology)
96
Elabscience Biotechnology cleaved poly
A The correlation analysis between TKT and γH2AX expression using the published proteomic and phosphoproteomics dataset of 159 HCC patients from Fudan University. B The expression levels of γH2AX in TKT high and low tissues from 6 patients with HCC were compared using WB. C Representative MIF staining micrographs showing TKT (low or high) and γH2AX expression in an HCC microarray. Scale bar, 200 μm (TKT, γH2AX, DAPI and Merged) and 20 μm (Enlarged). D The Hep3B (Left) and HCCLM3 (Right) cells were collected without treatment, or at 2 h, 6 h, 12 h and 24 h post 4 Gy X-Ray treatment with siCtrl or siTKT transfection. Cell lysates were immunoblotted with the indicated antibodies. M, protein ladder. E Representative fluorescence images (green, γH2AX; blue, DAPI) and quantification of γH2AX immunostaining in cells at the indicated time after X-Ray treatment with or without TKT depletion. The correlation analysis between TKT and RAD51 ( F ), <t>PARP1</t> ( G ), XRCC6 ( H ), XRCC4 ( I ) expression in HCC tissues by Spearman correlation analysis from TCGA database. Adapted from GEPIA: http://gepia.cancer-pku.cn/ . TPM, transcripts per million.
Cleaved Poly, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cleaved+parp+1/Cleaved-PARP1+(G215)+Polyclonal+Antibody/pmc10666272-59-8-33
Average 96 stars, based on 1 article reviews
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parp 1 antibody  (Elabscience Biotechnology)
96
Elabscience Biotechnology parp 1 antibody
A The correlation analysis between TKT and γH2AX expression using the published proteomic and phosphoproteomics dataset of 159 HCC patients from Fudan University. B The expression levels of γH2AX in TKT high and low tissues from 6 patients with HCC were compared using WB. C Representative MIF staining micrographs showing TKT (low or high) and γH2AX expression in an HCC microarray. Scale bar, 200 μm (TKT, γH2AX, DAPI and Merged) and 20 μm (Enlarged). D The Hep3B (Left) and HCCLM3 (Right) cells were collected without treatment, or at 2 h, 6 h, 12 h and 24 h post 4 Gy X-Ray treatment with siCtrl or siTKT transfection. Cell lysates were immunoblotted with the indicated antibodies. M, protein ladder. E Representative fluorescence images (green, γH2AX; blue, DAPI) and quantification of γH2AX immunostaining in cells at the indicated time after X-Ray treatment with or without TKT depletion. The correlation analysis between TKT and RAD51 ( F ), <t>PARP1</t> ( G ), XRCC6 ( H ), XRCC4 ( I ) expression in HCC tissues by Spearman correlation analysis from TCGA database. Adapted from GEPIA: http://gepia.cancer-pku.cn/ . TPM, transcripts per million.
Parp 1 Antibody, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cleaved+parp+1/Cleaved-PARP1+(D214)+Polyclonal+Antibody/pmc07485694-155-0-4
Average 96 stars, based on 1 article reviews
parp 1 antibody - by Bioz Stars, 2026-11
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cleaved parp  (Biorbyt)
91
Biorbyt cleaved parp
Figure 1. RSC96 cells were injured by high glucose (HG). RSC96 cells were cultivated in HG condition for 48 h. Cells in normal glucose (NG) were considered as con- trol. (A) Cell viability, (B) apoptosis rate, (C,D) cleavage <t>of</t> <t>caspase-3</t> and <t>PARP,</t> (E) ROS generation, and (F,G) expression of neurotrophic proteins were examined by CCK-8 assay, flow cytometry, Western blot and DCFH-DA probe. Data were presented as mean ± SD (n ¼ 3). p < .05 (Student’s t-test).
Cleaved Parp, supplied by Biorbyt, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cleaved+parp+1/Cleaved+PARP1+antibody/pm31581849-64-16-53
Average 91 stars, based on 1 article reviews
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anti parp1 antibody  (St Johns Laboratory)
90
St Johns Laboratory anti parp1 antibody
Figure 1. RSC96 cells were injured by high glucose (HG). RSC96 cells were cultivated in HG condition for 48 h. Cells in normal glucose (NG) were considered as con- trol. (A) Cell viability, (B) apoptosis rate, (C,D) cleavage <t>of</t> <t>caspase-3</t> and <t>PARP,</t> (E) ROS generation, and (F,G) expression of neurotrophic proteins were examined by CCK-8 assay, flow cytometry, Western blot and DCFH-DA probe. Data were presented as mean ± SD (n ¼ 3). p < .05 (Student’s t-test).
Anti Parp1 Antibody, supplied by St Johns Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cleaved+parp+1/Anti-Cleaved-PARP-1+(D214)+antibody/pm35705808-488-0-4
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anti-116 kda poly(adp-ribose) polymerase (parp) (1:1000 dilution) mouse monoclonal antibody that also detects the p85 cleavage fragment  (Becton Dickinson)
90
Becton Dickinson anti-116 kda poly(adp-ribose) polymerase (parp) (1:1000 dilution) mouse monoclonal antibody that also detects the p85 cleavage fragment
Figure 1. RSC96 cells were injured by high glucose (HG). RSC96 cells were cultivated in HG condition for 48 h. Cells in normal glucose (NG) were considered as con- trol. (A) Cell viability, (B) apoptosis rate, (C,D) cleavage <t>of</t> <t>caspase-3</t> and <t>PARP,</t> (E) ROS generation, and (F,G) expression of neurotrophic proteins were examined by CCK-8 assay, flow cytometry, Western blot and DCFH-DA probe. Data were presented as mean ± SD (n ¼ 3). p < .05 (Student’s t-test).
Anti 116 Kda Poly(adp Ribose) Polymerase (Parp) (1:1000 Dilution) Mouse Monoclonal Antibody That Also Detects The P85 Cleavage Fragment, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cleaved+parp+1/cleaved+parp1++d214++antibody/pmc08656492-99-28-31
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anti-116 kda poly(adp-ribose) polymerase (parp) (1:1000 dilution) mouse monoclonal antibody that also detects the p85 cleavage fragment - by Bioz Stars, 2026-11
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parp-1-dbd cdna  (Promega)
90
Promega parp-1-dbd cdna
Figure 1. RSC96 cells were injured by high glucose (HG). RSC96 cells were cultivated in HG condition for 48 h. Cells in normal glucose (NG) were considered as con- trol. (A) Cell viability, (B) apoptosis rate, (C,D) cleavage <t>of</t> <t>caspase-3</t> and <t>PARP,</t> (E) ROS generation, and (F,G) expression of neurotrophic proteins were examined by CCK-8 assay, flow cytometry, Western blot and DCFH-DA probe. Data were presented as mean ± SD (n ¼ 3). p < .05 (Student’s t-test).
Parp 1 Dbd Cdna, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A The correlation analysis between TKT and γH2AX expression using the published proteomic and phosphoproteomics dataset of 159 HCC patients from Fudan University. B The expression levels of γH2AX in TKT high and low tissues from 6 patients with HCC were compared using WB. C Representative MIF staining micrographs showing TKT (low or high) and γH2AX expression in an HCC microarray. Scale bar, 200 μm (TKT, γH2AX, DAPI and Merged) and 20 μm (Enlarged). D The Hep3B (Left) and HCCLM3 (Right) cells were collected without treatment, or at 2 h, 6 h, 12 h and 24 h post 4 Gy X-Ray treatment with siCtrl or siTKT transfection. Cell lysates were immunoblotted with the indicated antibodies. M, protein ladder. E Representative fluorescence images (green, γH2AX; blue, DAPI) and quantification of γH2AX immunostaining in cells at the indicated time after X-Ray treatment with or without TKT depletion. The correlation analysis between TKT and RAD51 ( F ), PARP1 ( G ), XRCC6 ( H ), XRCC4 ( I ) expression in HCC tissues by Spearman correlation analysis from TCGA database. Adapted from GEPIA: http://gepia.cancer-pku.cn/ . TPM, transcripts per million.

Journal: Oncogene

Article Title: TKT-PARP1 axis induces radioresistance by promoting DNA double-strand break repair in hepatocellular carcinoma

doi: 10.1038/s41388-023-02935-9

Figure Lengend Snippet: A The correlation analysis between TKT and γH2AX expression using the published proteomic and phosphoproteomics dataset of 159 HCC patients from Fudan University. B The expression levels of γH2AX in TKT high and low tissues from 6 patients with HCC were compared using WB. C Representative MIF staining micrographs showing TKT (low or high) and γH2AX expression in an HCC microarray. Scale bar, 200 μm (TKT, γH2AX, DAPI and Merged) and 20 μm (Enlarged). D The Hep3B (Left) and HCCLM3 (Right) cells were collected without treatment, or at 2 h, 6 h, 12 h and 24 h post 4 Gy X-Ray treatment with siCtrl or siTKT transfection. Cell lysates were immunoblotted with the indicated antibodies. M, protein ladder. E Representative fluorescence images (green, γH2AX; blue, DAPI) and quantification of γH2AX immunostaining in cells at the indicated time after X-Ray treatment with or without TKT depletion. The correlation analysis between TKT and RAD51 ( F ), PARP1 ( G ), XRCC6 ( H ), XRCC4 ( I ) expression in HCC tissues by Spearman correlation analysis from TCGA database. Adapted from GEPIA: http://gepia.cancer-pku.cn/ . TPM, transcripts per million.

Article Snippet: Antibodies against β-actin (AC038), PARP1 (A0942), Lamin B (A16909), FLAG (AE092), γ-H2AX (Ser139) (AP0099), GAPDH (AC001), HIS (AE028) were from ABclonal, while antibody against PAR (ALX-804-220-R100) was from Enzo Life Sciences.

Techniques: Expressing, Phospho-proteomics, Staining, Microarray, Transfection, Fluorescence, Immunostaining

A Coomassie staining of the TKT complex separated by SDS–PAGE. Hep3B cells stably expressing TKT-Flag were used for immunoprecipitation with an anti-Flag or anti-IgG antibody. TKT and TKT-interacting proteins, including PARP1, are indicated. The gel pieces containing regions of interest were analyzed by mass spectrometry. The list shows the peptides and coverage of TKT and PARP1 from the mass spectrometry. B Co-IP and WB analysis of the interaction between TKT and PARP1 in TKT-Flag and PARP1-His co-transfected Hep3B cells at different time points after X-Ray treatment. C Immunoblotting of indicated proteins in the in vitro pull-down assay of PARP1-His and TKT-Flag. D Coomassie staining of recombinant TKT, TKT-GFP and PARP1 protein (Left) and immunoblotting detection of PARylated TKT and PARP1 with the recombinant TKT, TKT-GFP and PARP1 protein (Right). E Co-IP and WB analysis of PARylated TKT in HCCLM3 and Hep3B cells in response to X-Ray. F , G Immunoblotting detection of PARylated TKT and PARP1 with or without PARP1 in an in vitro PARylation assay. H The activity detection of TKT and PARylated TKT by Transketolase Activity Assay Kit. Statistical analyses are presented as the mean ± s.d. from three independent experiments. * p < 0.05 of Student’s t test. The R5P abundance in DMSO or PARG inhibitor ( I ), PARP1 inhibitor ( J ), X-Ray ( K ) treated Hep3B cells. Statistical analyses are presented as the mean±s.d. from three independent experiments. * p < 0.05; ** p < 0.01; **** p < 0.0001 of Student’s t test. L , M Immunoblotting detection of PARylation in siCtrl or siTKT transfected HepG2 and HCCLM3 cells at different time points after X-Ray treatment.

Journal: Oncogene

Article Title: TKT-PARP1 axis induces radioresistance by promoting DNA double-strand break repair in hepatocellular carcinoma

doi: 10.1038/s41388-023-02935-9

Figure Lengend Snippet: A Coomassie staining of the TKT complex separated by SDS–PAGE. Hep3B cells stably expressing TKT-Flag were used for immunoprecipitation with an anti-Flag or anti-IgG antibody. TKT and TKT-interacting proteins, including PARP1, are indicated. The gel pieces containing regions of interest were analyzed by mass spectrometry. The list shows the peptides and coverage of TKT and PARP1 from the mass spectrometry. B Co-IP and WB analysis of the interaction between TKT and PARP1 in TKT-Flag and PARP1-His co-transfected Hep3B cells at different time points after X-Ray treatment. C Immunoblotting of indicated proteins in the in vitro pull-down assay of PARP1-His and TKT-Flag. D Coomassie staining of recombinant TKT, TKT-GFP and PARP1 protein (Left) and immunoblotting detection of PARylated TKT and PARP1 with the recombinant TKT, TKT-GFP and PARP1 protein (Right). E Co-IP and WB analysis of PARylated TKT in HCCLM3 and Hep3B cells in response to X-Ray. F , G Immunoblotting detection of PARylated TKT and PARP1 with or without PARP1 in an in vitro PARylation assay. H The activity detection of TKT and PARylated TKT by Transketolase Activity Assay Kit. Statistical analyses are presented as the mean ± s.d. from three independent experiments. * p < 0.05 of Student’s t test. The R5P abundance in DMSO or PARG inhibitor ( I ), PARP1 inhibitor ( J ), X-Ray ( K ) treated Hep3B cells. Statistical analyses are presented as the mean±s.d. from three independent experiments. * p < 0.05; ** p < 0.01; **** p < 0.0001 of Student’s t test. L , M Immunoblotting detection of PARylation in siCtrl or siTKT transfected HepG2 and HCCLM3 cells at different time points after X-Ray treatment.

Article Snippet: Antibodies against β-actin (AC038), PARP1 (A0942), Lamin B (A16909), FLAG (AE092), γ-H2AX (Ser139) (AP0099), GAPDH (AC001), HIS (AE028) were from ABclonal, while antibody against PAR (ALX-804-220-R100) was from Enzo Life Sciences.

Techniques: Staining, SDS Page, Stable Transfection, Expressing, Immunoprecipitation, Mass Spectrometry, Co-Immunoprecipitation Assay, Transfection, Western Blot, In Vitro, Pull Down Assay, Recombinant, Activity Assay

Figure 1. RSC96 cells were injured by high glucose (HG). RSC96 cells were cultivated in HG condition for 48 h. Cells in normal glucose (NG) were considered as con- trol. (A) Cell viability, (B) apoptosis rate, (C,D) cleavage of caspase-3 and PARP, (E) ROS generation, and (F,G) expression of neurotrophic proteins were examined by CCK-8 assay, flow cytometry, Western blot and DCFH-DA probe. Data were presented as mean ± SD (n ¼ 3). p < .05 (Student’s t-test).

Journal: Artificial cells, nanomedicine, and biotechnology

Article Title: Notoginsenoside R1 alleviates high glucose-evoked damage in RSC96 cells through down-regulation of miR-503.

doi: 10.1080/21691401.2019.1671434

Figure Lengend Snippet: Figure 1. RSC96 cells were injured by high glucose (HG). RSC96 cells were cultivated in HG condition for 48 h. Cells in normal glucose (NG) were considered as con- trol. (A) Cell viability, (B) apoptosis rate, (C,D) cleavage of caspase-3 and PARP, (E) ROS generation, and (F,G) expression of neurotrophic proteins were examined by CCK-8 assay, flow cytometry, Western blot and DCFH-DA probe. Data were presented as mean ± SD (n ¼ 3). p < .05 (Student’s t-test).

Article Snippet: Following primary antibodies against pro-caspase-3 (orb35276; dilution 1:1000), cleaved-caspase-3 (orb106556; dilution 1:1000), pro-PARP (orb88930; dilution 1:1000), cleaved-PARP (orb323122; dilution 1:2000), NGF (orb379540; dilution 1:1000), BDNF (orb519283; dilution 1:500), PI3K (orb395443; dilution 1:1000), p-PI3K (orb338965; dilution 1:500), AKT (orb29949; dilution 1:500), p-AKT (orb344403; dilution 1:1000), b-catenin (orb500798; dilution 1:1000) and b-actin (orb181785; dilution 1:500, Biorbyt, San Francisco, CA) were utilized in immunoblotting.

Techniques: Expressing, CCK-8 Assay, Flow Cytometry, Western Blot

Figure 2. Notoginsenoside R1 (NGR1) pre-conditioning ameliorated high glucose (HG)-evoked injury in RSC96 cells. (A) NGR1 with various dosages ranged from 10 to 100 lM was utilized to treat RSC96 cells. (B) RSC96 cells were pre-treated with NGR1 and then stimulated by HG. Cell viability was examined by CCK-8 kit. 50 lM was applied as optimum dosage for use in following experiments. (C) Apoptosis rate, (D,E) cleavage of caspase-3 and PARP, (F) ROS generation and (G,H) expression of neurotrophic proteins were examined by flow cytometry, Western blot and DCFH-DA probe. Data were presented as mean ± SD (n ¼ 3). p < .05 (ANOVA).

Journal: Artificial cells, nanomedicine, and biotechnology

Article Title: Notoginsenoside R1 alleviates high glucose-evoked damage in RSC96 cells through down-regulation of miR-503.

doi: 10.1080/21691401.2019.1671434

Figure Lengend Snippet: Figure 2. Notoginsenoside R1 (NGR1) pre-conditioning ameliorated high glucose (HG)-evoked injury in RSC96 cells. (A) NGR1 with various dosages ranged from 10 to 100 lM was utilized to treat RSC96 cells. (B) RSC96 cells were pre-treated with NGR1 and then stimulated by HG. Cell viability was examined by CCK-8 kit. 50 lM was applied as optimum dosage for use in following experiments. (C) Apoptosis rate, (D,E) cleavage of caspase-3 and PARP, (F) ROS generation and (G,H) expression of neurotrophic proteins were examined by flow cytometry, Western blot and DCFH-DA probe. Data were presented as mean ± SD (n ¼ 3). p < .05 (ANOVA).

Article Snippet: Following primary antibodies against pro-caspase-3 (orb35276; dilution 1:1000), cleaved-caspase-3 (orb106556; dilution 1:1000), pro-PARP (orb88930; dilution 1:1000), cleaved-PARP (orb323122; dilution 1:2000), NGF (orb379540; dilution 1:1000), BDNF (orb519283; dilution 1:500), PI3K (orb395443; dilution 1:1000), p-PI3K (orb338965; dilution 1:500), AKT (orb29949; dilution 1:500), p-AKT (orb344403; dilution 1:1000), b-catenin (orb500798; dilution 1:1000) and b-actin (orb181785; dilution 1:500, Biorbyt, San Francisco, CA) were utilized in immunoblotting.

Techniques: CCK-8 Assay, Expressing, Flow Cytometry, Western Blot

Figure 4. Notoginsenoside R1 (NGR1) pre-conditioning ameliorated high glucose (HG)-evoked injury in RSC96 cells through miR-503. (A) miR-503 mimic and its respective control (NC mimic) were transfected into RSC96 cells. Transfection efficiency was examined by qRT-PCR. The transfected or non-transfected cells were then treated by NGR1, HG or both. (B) Cell viability, (C) apoptosis rate, (D,E) cleavage of caspase-3 and PARP, (F) ROS generation, and (G,H) expression of neuro- trophic proteins were examined by CCK-8 assay, flow cytometry, Western blot and DCFH-DA probe. Data were presented as mean ± SD (n ¼ 3). p < .05 (ANOVA).

Journal: Artificial cells, nanomedicine, and biotechnology

Article Title: Notoginsenoside R1 alleviates high glucose-evoked damage in RSC96 cells through down-regulation of miR-503.

doi: 10.1080/21691401.2019.1671434

Figure Lengend Snippet: Figure 4. Notoginsenoside R1 (NGR1) pre-conditioning ameliorated high glucose (HG)-evoked injury in RSC96 cells through miR-503. (A) miR-503 mimic and its respective control (NC mimic) were transfected into RSC96 cells. Transfection efficiency was examined by qRT-PCR. The transfected or non-transfected cells were then treated by NGR1, HG or both. (B) Cell viability, (C) apoptosis rate, (D,E) cleavage of caspase-3 and PARP, (F) ROS generation, and (G,H) expression of neuro- trophic proteins were examined by CCK-8 assay, flow cytometry, Western blot and DCFH-DA probe. Data were presented as mean ± SD (n ¼ 3). p < .05 (ANOVA).

Article Snippet: Following primary antibodies against pro-caspase-3 (orb35276; dilution 1:1000), cleaved-caspase-3 (orb106556; dilution 1:1000), pro-PARP (orb88930; dilution 1:1000), cleaved-PARP (orb323122; dilution 1:2000), NGF (orb379540; dilution 1:1000), BDNF (orb519283; dilution 1:500), PI3K (orb395443; dilution 1:1000), p-PI3K (orb338965; dilution 1:500), AKT (orb29949; dilution 1:500), p-AKT (orb344403; dilution 1:1000), b-catenin (orb500798; dilution 1:1000) and b-actin (orb181785; dilution 1:500, Biorbyt, San Francisco, CA) were utilized in immunoblotting.

Techniques: Control, Transfection, Quantitative RT-PCR, Expressing, CCK-8 Assay, Flow Cytometry, Western Blot